Composite

Part:BBa_K3429010:Design

Designed by: Jan-Philip Kahl   Group: iGEM20_TU_Darmstadt   (2020-09-19)


TasA-sfGFP fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N-Terminus of sfGFP is linked to the C-terminus of TasA with the linker sequence GGGGSGGGGS; sfGFP has a His6-Tag; Restriction enzyme sites are located at the beginning and the end of this part and are suitable for the biobrick system


Source

synthetic

References